Part:BBa_K1199044:Design

HheCW249P 2,3-DCP(2,3-dichloropropanol)->Glycerol

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 697
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

No restriction site contained

Source

HheC is from Agrobacterium radiobacter

References

1 Jeffrey H. Lutje Spelberg, Johan E. T. van Hylckama Vlieg, Tjibbe Bosma, Richard M. Kellogg and Dick B. Janssen, A tandem enzyme reaction to produce optically active halohydrins, epoxides and diols, aDepartment of Biochemistry, Groningen Biomolecular Sciences & Biotechnology Institute, University of Groningen, bDepartment of Organic & Molecular Inorganic Chemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, 26 July 1999 .
2 Assis HMS, Sallis PJ, Bull AT, Hardman DJ. Biochemical characterization of a haloalcohol dehalogenase from Arthrobacter erithii H10a. Enzyme Microb Technol 1998;22:568–74
3 Lixia Tang, Xuechen Zhu, Huayu Zheng, Rongxiang Jiang, and Maja Majerić Elenkov, Key Residues for Controlling Enantioselectivity of Halohydrin Dehalogenase from Arthrobacter sp. Strain AD2, Revealed by Structure-Guided Directed Evolution, Appl Environ Microbiol. 2012 April